JCA061306-Construction of pSB3C6-J01093
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[p15A][CmR]+[pBad/araC][TraJF][dblTerm]
digest pSB1A2-J01093 (EcoRI/PstI/AlwNI, Neb2, Large-2100)
digest pAC997 (EcoRI/PstI, Neb2, Large-2028)
Ligate, Product is pSB3C6-J01093
Scheme failed, redesigned as:
PCR ca998F/BBa_G00101 on pSB1A2-J01093 (~2Kb, EcoRI/PstI, Neb2)
Sub into pAC997 (EcoRI/PstI, Neb2, Large-2028)
Ligate, Product is pSB3C6-J01093
