Imperial College/Courses/Spring2008/Synthetic Biology/Cellular And Molecular Biology Practical/Day 1

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• Introduction and overview
• Inoculate 2x5 ml overnight cultures

1. Aseptically put 2 x 5 ml of LB media into a 2 x 30 ml sterilin universal (pre-sterilised). Add 5 l of ampicillin antibiotic (50 mg/ml). (Question 1: What is the final concentration of ampicillin in the overnight culture ?)
2. Inoculate each 5 ml culture from a different single E. coli colony from the plate by picking and inoculating with a sterile loop.
3. Label each tube with your name and a number to differentiate each culture.
4. Place the cultures in the 28°C incubator for overnight growth.
5. Update your lab notebook.