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1% Agarose gel containing self-assembly reactions of dome and plate, both purified and unpurified as well as connection samples. All self-assembly reactions were performed in 12.5mM MgCl2 over a 17 hour non-linear ramp. A: 1) 1kb Ladder, 2) M13 control, 3) Unpurified 21nM plate, 4) 21nM plate purified with spin filter, 5) Unpurified 1nM dome, 6) 1nM dome up-concentrated, but not purified, using spin filter, 7) Up-concentrated and freeze n’ squeeze purified 1nM dome, 8) Connection attempt at room temperature, 9) Connection attempt at 2.5 hour linear ramp. B: The same gel, imaging the Cy3 emmision. Only the relevant bands are shown.