Image:Fixed AAP Varied H2O2.png

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Graph of absorbance over time for enzymatic activity assay of horseradish peroxidase (HRP). The concentration of HRP in all solutions was 2.3 μM and the signals of all solutions were amplified by adding 10 μL of 18 mM 4-iodophenol in DMSO. The concentration of AAP was fixed at 2.5 mM, while the concentration of H2O2 was varied between concentrations of 1.7 mM to 106.25 μM. For every 0.75 mL of H2O2 solution, 0.7 mL of AAP solution was added, for a total volume, including that of the 4-iodophenol solution, of about 1.46 mL in the cuvette.

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current21:16, 19 September 2012769×577 (33 KB)Melissa Novy (Talk | contribs) (Graph of absorbance over time for enzymatic activity assay of horseradish peroxidase (HRP). The concentration of HRP in all solutions was 2.3 μM and the signals of all solutions were amplified by adding 10 μL of 18 mM 4-iodophenol in DMSO. The concent)

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