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This picture is of the positive control with the +Fluo-3, AM. The magnification is 100X on mouse embryonic stem cells under FITC lighting. The intent of the procedure was to see the effect of histamine on the flourescence of these cells. Histamine would increase Ca2+ by opening ion channels in the cells and allowing Ca2+ to rush in due to a concentration gradient. This positive control is without the histamine, but flourescence can still be seen due to the free Ca2+ in the cells normally.
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|current||13:31, 16 March 2007||2,400×1,800 (813 KB)||W0iny (Talk | contribs)||(This picture is of the positive control with the +Fluo-3, AM. The magnification is 100X on mouse embryonic stem cells under FITC lighting. The intent of the procedure was to see the effect of histamine on the flourescence of these cells. Histamine would i)|
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|Camera model||FinePix4700 ZOOM|
|Date and time of data generation||00:38, 29 April 2000|
|Lens focal length||12 mm|
|Horizontal resolution||72 dpi|
|Vertical resolution||72 dpi|
|Software used||Digital Camera FinePix4700 ZOOM Ver1.00|
|File change date and time||00:38, 29 April 2000|
|Y and C positioning||2|
|Exposure Program||Normal program|
|ISO speed rating||200|
|Date and time of digitizing||00:38, 29 April 2000|
|Image compression mode||1.5|
|Maximum land aperture||3|
|Focal plane X resolution||2381|
|Focal plane Y resolution||2381|
|Focal plane resolution unit||3|
|Sensing method||One-chip color area sensor|
|White Balance||Auto white balance|