Image:DSCF0907.JPG

From OpenWetWare

Jump to: navigation, search

To explore various methods of fluorescent detection of Calcium, we either added Fluo-3 AM or transfected inverse-pericam into mouse embryonic stem cells, then treated some cells with histamine and others with cyproheptadine hydrochloride (Calcium mobilizing agents). Here is shown MES cells + Fluo-3 AM + 0.1 uM cyproheptadine hydrochloride @ 10x magnification. We see increased levels of Calcium as a result of adding cyproheptadine (untreated MES cells + Fluo-3 AM show significantly less fluorescence).

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeDimensionsUserComment
current13:52, 15 March 20072,400×1,800 (779 KB)Nzimm (Talk | contribs) (To explore various methods of fluorescent detection of Calcium concentration, we either added Fluo-3 AM or transfected inverse-pericam into mouse embryonic stem cells, then treated some cells with histamine and others with cyproheptadine hydrochloride (Ca)
13:45, 15 March 20072,400×1,800 (779 KB)Nzimm (Talk | contribs) (MES cells + Fluo-3 AM + 0.1 uM cyproheptadine hydrochloride)

The following page links to this file:

Metadata

This file contains additional information, probably added from the digital camera or scanner used to create or digitize it. If the file has been modified from its original state, some details may not fully reflect the modified file.

Personal tools