ISISBio:Internal/Lab Supplies/old

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Contents

August

Tues 26th August

  • ELGA UHQ5 RO cartridge, Cooling Fan, 1/2 day labour Quote WQ005499

Thurs 21st Aug

  • New England Biolabs BlpI R0585S 500 units 10,000 units/ml
  • Agilent - XL1 Blue Competent Cells 5 x 0.2ml ordered 2 packs
  • Promega BamH1 restriction enzyme
  • Fisher 5ml pipette tips
  • Jencons Jenway pH Meter expected end of August
  • 100pk 0.22um 25mm filter membranes

Weds 20th Aug

  • BioRad Precision Plus Protein Kaleidoscope standards x2

Date unknown

  • Bugbuster - ===arrived?===
  • Benzonase (from Novagen as well as bugbuster) ===arrived?===
  • Glucose (if we don't have some but I don't think we do) ===arrived?===
  • Fisher kilobase marker x2 one in stock one to follow
  • Fisher 25-500bp marker x2
  • Fisher Invitrogen Kanamycin sulphate 25g
  • NdeI and NaeI (Promega R7131 and R6801)
  • Merck dodecyl maltoside 25g

Mon 11th August 2008

  • Lyreco 10x Arch files for Louise's office R78
  • Whiteboard markers 1 x 4pk
  • Whiteboard cleaning and accessory kit
  • A4 ruled manuscript books x5

Fri 8th August 2008

  • Fisher by requisition fume hood and cabinet
  • items ordered Weds 6th August
  • Fisher Sodium Acetate 500g
  • 10ml syringes
  • 30ml Syringes
  • Paper rolls for lab
  • Fisher 25g Arabinose
  • 10pk 500ml Durans
  • 10pk 1L Durans
  • 10pk 250ml Durans
  • 5x 1ml Histrap Ni columns
  • ordered weds 30th July 2008 25g dodecyl maltoside (DM)
  • Weds 9th July 2008 arrived Heraeus Megafuge 11R, 230V Ex-Demo unit s/n 306090617 Delivery expected 11/08/08
  • T41 Swing out rotor
  • Set of 4 buckets for T41 rotor
  • Sealing lids for T40 buckets
  • Insert, 1 x 280ml (64 x 113mm)
  • Insert, 2 x 50ml (29 x 114mm)
  • Delivery BM sited
  • NH1/070 Custom underneath trolley delivery expected 31/08/08

Fri 1st August

  • GE Healthcare XK16/20 column x1
  • GE Healthcare 16/20 tube x1
  • NEB T4 DNA ligase x1
  • NEB Vent Polymerase x1

Weds 30th July

  • Fisher DTT x2
  • Fisher Cuvette Uvette 80 disposable single sealed x1 pack

Mon 28th July

  • ELGA service contracts and 6 month supplies
  • BioRad Short Plates, 5 2packs
  • BioRad Spacer Plates with 0.1mm integrated spacers 1pack

Tues 16th July 2008

  • Fisher NaOH 1M
  • Epp tips 2ul-200ul
  • Epp tips 0.1ul-20ul
  • Epp tips 100ul-1000ul

Mon 14th July 2008

  • HP Printer/copier/scanner and two colour cartridges -

Mon 7th July

  • Fisher LAB-850-112N Biohazard sign x4
  • Fisher LAB-850-113L Biohazard rigid sign x1
  • Fisher LAB-830-176B Now Wash hands sign x2
  • Fisher LAB-835-034X Hazardous Area sign x2
  • Fisher LAB-850-086L Flammable gas sign x1
  • ARCO Ergoclean Mop box with lid

Thurs 3rd July

  • NEB T4 DNA Ligase x2
  • NEB Vent DNA Polymerase

Tues 1st July

  • Fisher AKL-300-030M Foil 450mm x 75M x2
  • Fisher AUY-200-030K 9.5L sterilising pan x1
  • Fisher AUY-220-070H 3.1L sterilising pan x1
  • Fisher BPE-1760-25 Ampicillin sodium salt 25g x2
  • Fisher CRY-580-010X Cooler labtop for 12 tubes x1
  • Fisher FB55924 Cuvette UV single use 1.6ml 1 box
  • Fisher FB56209 Gel loading tips 1-200ul 1 box
  • Fisher FB71251 Cryobox with cardboard lid white 32mmx32mm 2packs
  • Fisher PDT-100-010M Incubation tray for petri dish
  • Fisher PMP-890-511T 100ul-1000ul pipettor tips eppendorf 5 boxes
  • ARCO Fliptop bin blue 54L x1
  • ARCO Fliptop bin red 54L x1
  • Ergoclean mop handle 2.5M long x1
  • Ergoclean Wet multifibre mix head x1

JUNE

26th June

  • Amazon HP Ink Cartridge Tricolour x2
  • Amazon Photosmart C3180 All-in-one Printer

24th June

  • Fisher SAT-670-010M Spillage clean up 3M chemical sorbent sheets 4 boxes
  • Perbio from Thermofisher 23225 Bradford Assay Kit 500rx x1

23rd June

  • Merck 324355 DM 25g
  • Promega R6801 Nde1
  • Promega R7131 Nae1
  • Fisher BPE-2553-100 Kilobase Marker
  • Fisher BPE-LAD4-200 DNA MW Markers 25-500bp
  • Fisher Invitrogen VX11815032 Kanamycin Sulphate
  • Bradford reagent or stuff to make it - have made enquiries. Should be ordered Fri 13th.
  • Welded 2 tier trolley
  • Sterile pipettes 5ml
  • Sterile pipettes 10ml
  • Sterile pipettes 25ml
  • Sterile pipettes 50ml
  • Rhamnose (sugar - Fisher 17408-0250 for 25 g)
  • BioRad- Bio-Safe Coomassie Stain, 5L x1 A box with a tap on for easy dispensing
  • BioRad- Precision Plus Kaleidoscope Standards 500ul (50 applications at 10ul per well) x2 This is a rainbow marker which I used at Reading Uni and was brilliant because there is never a doubt about the size of each marker band. I'v ordered 2 so we can use one and when that is used up we re-order so we never run out. To be stored at -20 when not in use. Store at room temp or in cold room when in use.
  • Syringe filter sterile 0.2um 25mm
  • Syringe filter sterile 0.2um, 4mm
  • Vivaspin protien concentrators 0.1-0.5ml
  • Merck Dodecyl Maltoside 5g, overnight shipment requested
  • -80 freezer storage-fisher

Weds 4th June

  • Ep tips box 0.1-20ul

===MAY

Fri 30th May

Thurs 29th May

  • Fisher Revco racking x3

Weds 28th May

Tues 27th May

Mon 26th May

Fri 23rd May

BioRad Biosafe Coomassie Stain 5L

Thurs 22nd May

Weds 21st May

Tues 20th May

  • Novagen Rosetta2 pLyS (cat# 71401)
  • Promega KRX Competent cells (20 x 50 uL - cat# L3002)
  • Fisher 15ml tubes
  • Fisher 50ml tubes
  • Lab coats XXL light blue x3
  • 0.1ul-20ul tips in box x3
  • 100ul-1000ul tips in box x4
  • 5ml tips in box x2
  • 2ul-200ul tips in box x4

Mon 19th May

13 May

  • Ammonium Sulphate 2x500g
  • IPTG 2x10g
  • 4L flask x8
  • Face Shield sign x2
  • Face Shield x2
  • XL lab coat x6
  • XXL lab coat x3
  • HiTrap Desalting column 5 × 5 ml 17-1408-01 £112.00 GE life sciences
  • dodecyl maltoside 5g

arrived (ordered 1st May 2008)

  • BPE1422-2 Yeast Extract 2kg x1
  • BPE-310-500 HEPES Crystals 500g x1
  • BPE328-1 Sodium Hydrogen Carbonate x1
  • CRY-190-010Q Cryobox 10pk x2
  • FB50323 Weighing boat 5ml 1000pk x1
  • FB50325 Weighing boat 30ml 1000pk x1
  • FB50327 Weighing boat 100ml 1000pk x1
  • FB50377 Weighing boat 250ml 1000pk x1
  • CRY-595-030P Centrifuge tubes 35ml 10pk x2

arrived 22nd May 2008

  • 50 ml Falcon Tubes
  • 15 ml Falcon Tubes
  • Petri Dish Rack


APRIL

ordered 11th April 2008 Fisher order 7515525 1104081

  • 10pk 500ml beakers
  • 2 x 5pk 1L beakers
  • 10pk 250ml beakers ordered but out of stock til May!
  • 10pk 100ml glass beaker
  • 10pk 250ml glass beaker
  • 10pk 600ml glass beaker
  • 3x 1L glass beaker
  • 2 x 1000pk PCR tubes 0.2ml
  • 1.5ml tube float rack 4pk
  • 0.5ml tube float rack 4pk
  • 25g low melting agarose
  • 5 thermometers -10 to 110 degrees
  • 0.5ul-10ul tips 10 x 1000pk ordered but out of stock til May!

ordered 7th April 2008 Screw Fix

  • 3 shelf units for cold room

ordered 2nd April 2008

  • Fisher Incubator Shelf & 2 Supports x2 ING-205-502L

Week 2

11th April 2008

  • ELGA cartridges LC148, LC163, LC181, CT all x2

10th April 2008

  • Steps/ladders from screw fix

9th April 2008

  • Fisher 100 mg anhydrotetracycline (not Louise's GPC)

8th April 2008

Screw Fix

  • Trolley for lab move and/or fermentor
  • Spirit level for lab
  • 6 containers for transporting bottles across lab
  • Mobile step for office

Week1

3rd April 2008

  • Fisher 5L Beakers x5 FB33119 £11.74 each
  • Fisher Magnetic Retriever x1 FB55669
  • Fisher Stirrer fleas 10pk x1 FB55601
  • Fisher Stirrer fleas 10pk x1 FB55591


MARCH

31th March 2008

ordered 26/03/08

  • BPE2543-2 Thrombin, bovine plasma Fisher BioReagents from Fisher Scientific
  • Merck- n-Dodecyl-b-D-maltoside, ULTROL® Grade Cat. No. 324355 5g

28th March 2008

ordered 27/03/08

  • Fisher- Protein Concentrators 2ml 10KMW Millipore
  • Fisher- Containers for agar plates

27th March 2008

  • Ultra violet DNA gel lamp and spares
  • DNA marker (e.g. Fisher BPE2553-100)
  • GE Healthcare XK 16/20 column 18-8773-01
  • GE Healthcare HiPrep 16/10 DEAE-FF 17-5090-01

20th March 2008

  • ordered 07/03/2008
  • 3kg balance - Fisher
  • 0.5ul-10ul tips 1000pk - Fisher

13th March

  • ordered 12/03/2008
  • T4 Polynucleotide kinase - recombinant from E.Coli Fisher BioReagents 100 U BPE3212-1 £23.50 Fisher Scientific
  • PCR tube Fisherbrand thin walled domed cap polypropylene 0.2mL natural pack of 1000 FB68720 £64.60 Fisher Scientific x2

These inc VAT £131.55 Ref.7482996 Gail x2234

  • ordered 11/03/2008
  • Fisher GHL-415-010D gas burner lighter x2
  • Fisher FB67020 pH Electrode x1
  • Fisher FB70260 Timer x6
  • Fisher BMD-110-021W Batteries x6
  • Fisher LAB-550-010W white tape x1
  • Fisher LAB-551-010Q Orange tape x1
  • Fisher LAB-553-010E Red tape x1
  • Fisher LAB-555-010E Yellow tape x1
  • Fisher LAB-557-010D Green tape x1
  • Fisher LAB-559-010X Blue tape x1
  • Fisher LAC-730-010K Black pen 10pk x1
  • Fisher LAC-730-020H Blue pen 10pk x1
  • Fisher LAB-340-030Q Round labels 1000pk x1
  • Fisher HW1-LB-S small lab coat x9
  • Fisher HW1-LB-M Medium lab coat x9
  • Fisher FB55301 5pk Clamp set
  • Fisher FB68403 Base for clamp x2
  • Fisher FB55305 Bosshead Clamping screws 5pk x1
  • Fisher FB34531 1-200ul tips 1000pk x10
  • Fisher FB34511 0.5ul-10ul tips 1000pk x10
  • Fisher FB34611 200-1000ul tips 1000pk x10
  • Fisher FB70173 cleaning detergent
  • Total of order inc VAT £1367.22

12th March

  • Sigma low range molecular weight markers Sigma 6.5 - 66 kDa M3913-10vl x1
  • Sigma Mid range molecular weight markers Sigma 6.5 - 200 kDa S8445-1vl x4
  • Sigma high range molecular weight markers Sigma 36 - 20 kDa S8320-10vl x1
  • Sample buffer solution Sigma S3401-10vl x2
  • LDao (n,n-Dimethyldodecylamine n-oxide) Sigma D9775-SG x1
  • Spatulas 0.09inch tip Sigma Z243213-2EA £11.80 x10

11 March

  • ordered 10/03/2008
  • Promega Wizard Gel and PCR Clean up Kit 250rx A9282 x1
  • GEHealthcare - PEEK tubing 1/16", i.d. 0.75 mm 2 M 18-1112-53 1 £50.50 £50.50
  • Superloop, 1/16" fittings (ÄKTAdesign) 10 ml 1 EA 18-1113-81 1 £360.00 £360.00 Sub Total: £410.50
  • n-dodecyl beta-D-maltose D4641-SG - Merck
  • cAMP-Merck
  • BL21 (DE3) competent cells -Merck

10 march

  • 2 x 100g electrophoresis agarose - Fisher
  • 1Kg Ammonium Acetate - Fisher
  • 1ul-200ul tips 1000pk - Fisher
  • 250ul-1000ul tips 1000pk - Fisher
  • 3.5 KDa cut off dialysis tubing
  • PCR thermocycle control panel
  • Miniplasmid kits
  • Acetic acid
  • DTT
  • glycine
  • sodium bicarbonate
  • EDTA
  • chloramphenicol
  • butane cartridges for bunsen
  • ethidium bromide
  • kanamycin sulphate
  • ampicillin
  • 0.45 u sterile filters

4 march

  • Top 10 cells
  • Arabinose


FEBRUARY

25 Febuary=

  • Yeast extract
  • 0.22 um u sterile filters
  • X-gal
  • KCl
  • KH2Phos
  • Na2Hphos
  • Triton X-100
  • biohazard autoclave gloves
  • bunsen burner
  • batteries
  • orange bins
  • SDS

20 February

  • 1000 MWCO Spectrapor dialysis tubing
  • ammonium acetate
  • lysozyme
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