IGEM:metu/2009/Notebook/wound dressing/2009/09/07

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07.09.2009 1. Isolations of 4.07,2.09,1.09,2.07,5,1.07,8.07,6,10M Lysis,7,9,BB were made. 2. Digestions were made. The following amounts are added to microtubes; 1.07,1.09 , Buffer Tango 2 µl Spe1 2 µl DNA 16 µl =20 µl 2.07,2.09 , Buffer O 2 µl Pst1 2 µl DNA 16 µl =20 µl 1.09 control, Buffer Tango 1 µl Spe1 0 µl DNA 9 µl =10 µl BB,2.09 control, Buffer O 1 µl Pst1 0 µl DNA 9 µl =10 µl