IGEM:metu/2009/Notebook/wound dressing/2009/08/26

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Image: Wound dressing logo.jpg

26.08.2009

1. Isolations of 5,6,8,10 and storages of 2,3,4,5,6,7,8,9,10,11 were made.

2. We tried to make digestions but we couldn't. The following amounts are added to microtubes;

    6,        water           9.4  µl                 18.4µl 
              FD Buffer       2  µl                    3 µl
              Spe1            3 µl          EcoR1      3 µl      
              DNA             5.6 µl                   5.6 µl
    8,        water           8.9µl                   17.9 µl
              FD Buffer       2 µl                     3 µl
              Xba1            3 µl           Pst1      3 µl      
              DNA             6.1µl                    6.1 µl
 

2nd part,

          solution  30 µl
          enzyme    4 µl
          buffer    4 µl
          water     2  µl   =40µl

3. Outcomes of nanodrop;

                 ng/  µl             Abs
          5      578.4              4.563  
          6      531.3              5.168
          8      489.6              4.649
         10      290.2              2.967

4. Electrophoresis order;

  1) leader / c5 / 5 / empty / 10 / c10 / leader
  2) leader / 5 / 5 / c5 / c10 / 10 / 10 / leader




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