IGEM:metu/2009/Notebook/wound dressing/2009/07/13

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13.07.09

1. Storage of transformed cells.

Pick single colonies with toothpick and inoculate in 5 ml LB medium (broth)  containing  appropriate antibiotic. (ampicilin) 
Amp = 5 µl.Incubator  240 rpm, overnight (16 hours)
Centrifuge at 4000 rpm for 10 min.
Remove supernatant.
Resuspend pellet with 1 ml LB
15% sterile glycerol ( 1ml × %15 = 150 µl)
Store at -80 C.
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