IGEM:Wisconsin-Madison/2009/Notebook/Spring Training 2012/2012/02/29

iGEM Project name 1

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Sequencing the pfu plasmid for iGEM cloning training cleanSEQ of Andrew's pfu plasmid using Matt's pET Up primer and Andrew's T7 terminator reverse primer 10 ul Big Dye reaction 1.5 ul Big dye buffer 1 ul Big dye 1 ul primer 1 ul DNA (171 ng/ul pET1a pfu) 5.5 ul water "seq50" on iGEM block A 95°C for 3:00 51 repeats of: 95°C for 0:30 50°C for 0:10 60°C for 4:00 Cleaned up and eluted in 20 ul nuclease free H2O.