IGEM:Virginia United/2009/Notebook/2010 VT Lab/2010/07/26

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July 26th, 2010 Met with Synthetic Biology Research Group Located supplier and placed order with Julie for E. coli strain with the copper efflux gene knocked out Ran two gels on purified DNA from gel bands Received sequence of copper construct sample from UVA subteam and compared to design sequence to determine if construct was present in PCR product Worked on mathematical modeling Started overnight broth cultures of cotransformed (RFP and GFP) cells in LB carbenicilin and kanamycin, TB carbenicillin and kanamycin, and LB with no antibiotics Measured OD of overnight cultures (above) at 6.5 hours growth Started overnight broth cultures of TOP10, EGFP, ACGFP, and DSRED cells for spectral unmixing Uploaded journal entries onto iGEM WIKI Autoclaved glassware, supplies, and biohazard waste