July 15th, 2010
- Prepared E. coli with copper solutions in wider flasks for better oxygenation
- Retested copper promoter constructs made from overnight cultures the night before and incubated at 37C for 4 hours
- Aliquoted 1ml of the copper sulfate treated cultures to microcentrifuge tubes, centrifuged, and observed pellets for fluorescence with UV lamp
- Compared optical density readings of the Copper sulfate treated cultures using various available spectrophotometers (Perkin Elmer Lambda 20, Thermo H, plate reader)
- Rechecked RBS efficiencies of fluorescent proteins using RBS calculator
- Discussed project description alterations
- Discussed different wiki’s and own ideas for the site
- Made more liquid cultures of E. Coli
- Worked on web page design for the wiki
- Investigated approaches to eliminate copper from media: Chelex, EDTA, M9 Minimal media
|
iGEM Project name 1
Main project page
Previous entry Next entry
