IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/06/17

Week 4

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(6/17 – 6/23) Attempted electrotransformation of Bordatella to electroporate DNA into the cells. This did not work. We found that RB50 and RB53 are not electrotransformable Used Qiagen Viral RNA kit to purify phage genome and then did nanodrop successfully. Also performed gel electrophoresis to visualize size and quantity of DNA. Performed PCR on isolated phage genome BPP Gel electrophoresis image confirmed that the start and end of the BPP phage genome were what we thought they were. The 260/280 ratio for nanodrop of the genomic phage DNA isolation was off. It needed to be closer to 1.8. We attempted isolation of the hCG plasmid from DH5-alpha using Qiagen prep spin miniprep kit.