August 12th, 2011
- Divided a URA DO plate into four quadrants and streaked each quadrant with one transformed yeast colony to provide further purification of the yeast collected. Plates were parafilmed and incubated at 25C.
- Troubleshooting 13-22 pca reaction...
Ran all of the single oligos 13-62 on a gel to make sure the oligos exist:
1. 13
2. 14
3. 15
4. 16
5. 17
6. 58
7. 59
8. 60
9. 61
10. 62
- Oligos 16, 17, 58, and 59 appeared as very faint bands. This means that we have a lot less DNA in the samples than we expected, which could have resulted in our failed pca reactions. [no image]
- Ran 4 pca reactions: 13-58, 13-16, 17+58, 59-62. Ran the samples on the gel with the oligos that appeared faint in the other gel:
1. ladder
2. 16
3. 17
4. 58
5. 59
6. 13-58
7. 13-16
8. 17+58
9. 59-62
- Still can’t see the oligos, but we have clear bands for 13-58 and 59-62. So we decided to run a second set of pca reactions. [no image]
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