June 14th, 2011
- Performed MiniPrep on liquid culture of bacteria from one of the two falcon tubes and put 6 microcentrifuge tubes of resultant DNA into cold storage. Made significant progress on sequence, struggled with poly-A tail problem until we decided to just order an ultramer that would cost a bit more, wrote simple script to divide sequence into 60mers for Gibson assembly. Checked on yeast liquid cultures from the wheel in Kozminski’s lab, found no cell growth, attributed this to failure to no glucose in the YPD mixture. Found our access codes won’t work for the NanoDrop computer and emailed the administrator in charge.