IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/07/26
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July 26, 2010
EGFP has stayed elusive to cloning with PCR so we tried it again. Also we ran out of MicF and had to clone that again. We ran two reactions:
The PCR setting that was used:
Numbers 2-4 were repeated 32 times.
I also inoculated 1 white colony from each of the 4 plates made on 7/22/10. Each was inoculated into 5 mL of LB Broth with added 5 uL of CAP resistance added to them. They were then put into a spinner in the 37 C room overnight.