IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 RNA Decoder/2010/07/01
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July 1, 2010
We ran another PCR for MicA, MicF, OmpA, OmpF, and Hfq. We also ran two controls for the experiment: one without polymerase and one without template. Their uL amounts were replaced with dH2O. The procedure we followed:
A master mix instead of adding each of those amounts to each tube. The master mix was made as such (MM for 5 RXNs):
We then ran the PCR at these settings:
Once the PCR was complete we ran a gel for the trials.