7/14/10
Transformation
- Transformed 2μL of plasmids from well 12K, Plate 1 of the Spring 2010 DNA Distribution into a 100μL solution of competent cells.
- Used new electroporation cuvette, which seemed to be differently shaped than old ones. (May have to use more solution for an efficient transformation)
- Added 1000 μL LB broth, mixed, and placed cuvette labelled "GFP" into 37°C room for 1 hour.
- Also placed 1 Amp and 1 Kan plate into 37°C room for 1 hour.
Plating
- Spread 100μL of GFP transformed cells onto Amp plate and Kan plate from previous transformation.
- Placed plates into 37°C room overnight.
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iGEM Project name 1
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