IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Gold Bioremediation/2010/08/13

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August 9th to August 13th

Amanda and Tom:
Monday: digestion, gel extraction, ligation of gesABC to psb1c3, PCR for verification of golB, inoculated golB colonies, digested/ligated golT and pgolS
Tuesday: verifying golb + golS with digestion of miniprep, transformed and plated gesabc, golt, pgols, pulled out ecfp, eyfp, rbs, terminator from glycerol stock, lab meeting
Wednesday: games camp, ligating golt, golb, and gols to terminator, ligating ges into plasmid, verification pcr of golb+s, added bsaI sites to golb
Thursday: miniprepped pgols, golt, digestion of minipreps, gel

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