IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/09

UIUC - Arsenic Collection System!

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9/9/10 12:30 PM Verification PCR Ingredient Amount 10X Buffer 2 μL 10 mM dNTPs .4 μL Template 1 μL (20 ng) F Primer .4 μL R Primer .4 μL Polymerase .2 μL H2O 15.6 μL Samples: B0034ArsRSDM2B0015pSB1C3 negative control DI H2O Primers: VF2, VR, 20 micromolar Program: 95°C 3 min 95°C 30 sec 55°C 30 sec 72°C 1 min go to step 2 30X 72°C 3 min hold at 4°C Farthest lane to the right contains negative control. Lane to the left of the furthest lane is the ArsR sample. Remaining lanes are Tom's. 1%, TAE, 160V