IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/09/03

UIUC - Arsenic Collection System!

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9/3/10 8:55 AM Growth on ArsR-B0015-pSB1A2 plate. good streaks on all plates except B0015 on Amp (no growth)--B0015 grew on Amp but not Kan even though the resistance should be Amp/Kan... 10:00 AM Gel Lane Sample Amount 1 1 Kb Ladder 1 μL 2 ArsB 1 4 μL dye, 20 μL sample 3 ArsB 2 4 μL dye, 20 μL sample 4 ArsB 3 4 μL dye, 20 μL sample 5 ArsB 4 4 μL dye, 20 μL sample 6 Negative Control 4 μL dye, 20 μL sample 3:00 PM Made 5 mL cultures (3X of ArsR-B0015) Re-streaked B0015, B0034 and gas vesicles.