6/27/10
9:10 AM
miniprep (promega kit):
lost about 2.5 mL of colony 2 for ArsR ligations again!
used new neutralization solution since there were chunks floating in the old solution.
concentrations:
- B0015 pSB1AK3: 200 ng/μL
- B0034 pSB1A2: 232.2 ng/μL
- pSB1C3 RFP: 279.1 ng/μL
- B0034-ArsRSDM1-B0015-pSB1C3 3 (1'): 339.2 ng/μL
- B0034-ArsRSDM1-B0015-pSB1C3 4 (2'): 252.6 ng/μL
Glycerol stocks:
- pARsRF1R2 1' ISB218
- pARsRF1R2 2' ISB219
3:00 PM
Dilutions
Primers diluted to 10 micromolar
samples diluted to 20 ng/μL
Sample |
concentration |
Sample Amount |
H2O amount |
Total Vol. |
VF2 |
100 micromolar |
10 μL |
90 μL |
100 μL |
VR |
100 micromolar |
10 μL |
90 μL |
100 μL |
B0034-ArsRSDM1-B0015-pSB1C3 1 |
767 ng/μL |
.3 μL |
9.7 μL |
10 μL |
B0034-ArsRSDM1-B0015-pSB1C3 2 |
376 ng/μL |
.53 μL |
9.47 μL |
10 μL |
B0034-ArsRSDM1-B0015-pSB1C3 3 (1') |
339.2 ng/μL |
.59 μL |
9.41 μL |
10 μL |
B0034-ArsRSDM1-B0015-pSB1C3 4 (2') |
252.6 ng/μL |
.79 μL |
9.21 μL |
10 μL |
LamBF1R 1' |
180 ng/μL |
1.11 μL |
8.89 μL |
10 μL |
LamB F1R 2' |
145 ng/μL |
1.38 μL |
8.62 μL |
10 μL |
LamB F2R |
68 ng/μL |
2.94 μL |
7.06 μL |
10 μL |
LamB F2R Green |
285 ng/μL |
.7 μL |
9.3 μL |
10 μL |
pARsR F1R2 1' |
342 ng/μL |
.58 μL |
9.42 μL |
10 μL |
pArsRF1R2 2' |
378 ng/μL |
.53 μL |
9.47 μL |
10 μL |
pArsR F1R2 1 |
247 ng/μL |
.81 μL |
9.19 μL |
10 μL |
pArsR F1R2 2 |
456.2 ng/μL |
.44 μL |
4.56 μL |
10 μL |
|