IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/07/02

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7/2/10

1:00 PM

PCR

Ingredient Amount Mastermix (6 reactions)
10X buffer 4 ul 24 ul
10 mM dNTPs 1 ul 6 ul
Template 1 ul 6 ul
Primers (F/R) 4 ul added to each reaction individually
Polymerase .4 ul 2.4 ul
H2O 29.6 ul 177.6 ul

add 36 ul mastermix to each reaction, 4 ul primers for a total 40 ul reaction.

Program:

95 °C 5 min
95 °C 30 sec
55 °C 30 sec
72 °C 2 min
go to step 2 31X
72°C 5 min
hold at 4°C

Samples:

1. PAL

2. ArsB

3. PAL negative control (no primers)

4. ArsB negative control (no primers)



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