IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/28
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6/28/1011:00 am: PCR of backbones: 10x Buffer 2 ul dNTPs .5 ul Template 1ul Polymerase .2 ul Primer F 1 ul Primer R 1ul diH2O 14.3 ul Total: 20 ul Template: pSB1A2, pSB1C3, pSB1T3, pSB1AC3-2, pSB1AK3 Psb1AT3. Negative Controls: 1. no template, 1 ul water instead 2. For each sample, no polymerase, .2 water instead. Program: iGEM 2Kb 11:35 am Diluted primers VF2 and VR to 20 um, PCR: used 100ng template for each reaction Program: iGEM 2Kb
1:15 PM digestion for Steve RFP <html> <table> <table border="1" cell padding="7"> <tr> <td> XbaI </td> <td> 1 ul </td> <tr> <td> PstI </td> <td> 1 ul </td> <tr> <td> Buffer 2 </td> <td> 5 ul </td> <tr> <td> BSA </td> <td> .5 ul </td> <tr> <td> H2O </td> <td> 41.5 ul</td> </tr> <tr> <td> DNA </td> <td> 1 ul</td> </tr> </table> </html> Lock1
key1
pBAD
2:30 PM gel GRAB PICTURE FROM COMPUTER
3:30 PM GRAB PICTURE FROM COMPUTER Gel of Backbones Top half of gel
Bottom half of gel
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