IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/05/25

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5/25/10

Bootcamp Day 2

Digestion/Ligation

Amount for 500ng DNA

120 4.26 ul

12M 2.69 ul

J23100 2.17 ul (promoter)

BBa_J04450 1.76 ul (backbone)

Post-Ligation Concentrations

J23100-12M-BBa_J04450 312.6 ng/ul

J23100-12O-BBa_J04450 302.13 ng/ul

Transformations/Plating

For both 12O and 12M constructs:

50 ul transformed cells on chloramphenicol plate

100 ul transformed cells on chloramphenicol plate



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