IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/05
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Completed the chromosomal preps of 10 of the samples, then ran in a PCR for 3 hours following standard volumes for the master mix with Taq polymerase, for 20ul in each tube. Made 4 flasks of 500ml SFM (500 ul Ny per 500 ml). Autoclaved for 1 hr and poured into plates.
Checked the plates growing the cloned GUS vector, which did not appear to work, so repeated PCR with GUS template and restriction sites (Nde and Spe) using 8x 20ul volumes with standard phusion mix. Run on a thermocycler PCR. Instead of cloning into pMS82, it will be cloned into pAU3-45.
More spore stocks prepared.