IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/06
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Today we checked our plates which we had prepared the day before for colonies. We expected the colonies to have a red colour as part BBa_J04450 encodes red fluorescent protein. This was not the case on first inspection, but after a couple more hours incubation, the colour began to show.
Satisfied that the competent cells were efficient, we began another transformation of AntGpNeo DNA into our α-Select competant cells and plated the sample on Agar plates with Ampicillin. We also prepared two other plates with the plasmids PUC19 and PGEM32 for comparison. The plates were incubated overnight.