IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/14
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De-replication of single Streptomyces colony plates where it appeared we had duplicates.
As the BL21 plates were unsuccessful, we decided to make some new competent cells, following the protocol. These cells were then transformed with Pet28AntA and spread onto plates for incubation overnight at 37°C.