IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/08

Week Ten

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Floor Two A restriction enzyme digest was prepared using Nde1 and Xba1 to characterise our biobricks. The samples were analysed using agarose gel electrophoresis. The gel was stained with Etbr, this allowed us to define the smaller bands, but the marker become unclear fig 1. Fig 1: Staining with Ethidium Bromide the gel from the analysis by restriction enzyme digest of the biobricks with Nde1 and Xba1 enzymes. Lanes 1-9 containing samples 3b1, J04450, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2, 2b1, 2b2, 2c1 respectively. We began entering information about our biobricks into the registry