IGEM:University of Debrecen:Making petri dishes

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<a name="OLE_LINK5"></a><a name="OLE_LINK4">Scientific background<o:p></o:p></a>

A Petri dish (or Petri plate or cell culture dish) is a shallow glass or plastic cylindrical lidded dish that biologists use to culture cells. It was named after German bacteriologist Julius Richard Petri, who invented it when working as an assistant to Robert Koch. Glass Petri dishes can be reused by sterilization (for example, in an  autoclave or by dry heating in a hot air oven at 160°C for one hour). For experiments where cross-contamination from one experiment to the next can become a problem, plastic Petri dishes may have to be disposed of after one use.<o:p></o:p>

Modern Petri dishes often have rings on the lids and bases which allow them to be stacked so that they do not slide off one another. Multiple dishes can also be incorporated into one plastic container to create what is called a "multi-well plate".<o:p></o:p>

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Material<o:p></o:p>

·         Plain gelatin<o:p></o:p>

·         Water<o:p></o:p>

·         Sugar<o:p></o:p>

·         Beef bouillon granules or cubes<o:p></o:p>

·         Foil muffin or cupcake pan cups<o:p></o:p>

·         Muffin pans<o:p></o:p>

·         Ziploc bags<o:p></o:p>

·         Sauce pan and stove<o:p></o:p>

·         Refrigerator<o:p></o:p>

·         Measuring spoons<o:p></o:p>

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Procedure<o:p></o:p>

Step1  <o:p></o:p>

Be sure that all utensils that will be used are sterilized. Place the muffin cups in the muffin pans without touching the inside of any of the papers.<o:p></o:p>

Step 2<o:p></o:p>

Mix 4 packages of gelatin, 4 cups of cold water, 8 tsp. <a href="http://www.ehow.com/how_5326576_make-homemade-petri-dishes.html" target="_blank">sugar</a>, 4 tsp. bouillon or 4 cubes in a sauce pan. Slowly bring these ingredients to a boil while continually stirring. Pour the hot mixture into the muffin papers until about half full.<o:p></o:p>

Step 3<o:p></o:p>

Cool until the gelatin mixture is solid. Protect the mixture from any contaminants during the cooling process.<o:p></o:p>

Step 4<o:p></o:p>

<a name="OLE_LINK7"></a>Once cool, place each cup liner into a fresh Ziploc baggie. Continue to be careful not to touch the inside of the muffin papers or the mixture itself. Keep the individually packaged petri dishes in the refrigerator until it is time to use them.<o:p></o:p>




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