IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/08/13

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August 13th, 2009 measured DNA conc. using nanodrop appartus. this DNA was cut using PacI and AscI (overnight) and then gel purified. Ligation (super GFP into Longtine Plasmids [Kan and His]) 20 ul RXNs 4 5 x buffer 2. plasmid 2. insert 1 igase 11 ddH2O Ran four reactions 1. S-GFP + His Plasmid 2. S-GRFP + kan plasmid