IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/08/10
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August 10th, 2009Run PCR to confirm genomic integration at specific promoter regions. Dilute Primers for this Restreak E.coli onto LB/AMP for DNA replication
PCR Design 25 ul RXNs, on bead, one blot of cells PCR Tube #s
Run PCR using superfolder E-COli as template. Hopefully get superfolder fragments which can be ligated onto the longtin plasmid. If not, I innoculated a couple of Falcon tubes of LB/AMP with superfolder E.Coli |