IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/22
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July 22nd, 2009
- Used 3 ml cultures as starter
- diluted to OD 6, 3 ml total reaction
- Pick up at 2 PM. Add 50 ul of 20 mM paraoxon to 950 ul culture (OD 0.2)
- incubate @ 30 degrees C 1 hour
- Made slides. viewed under fluorescent microscope in Kron lab.
- Found green cells in...
- 35-2 - 35-5 - 346-2 (check)
pictures are in microscope room on knapp side of building.
- western blot to quantify?
End of the Day