IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase/2009/07/16

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Thursday, July 16, 2009

Yeast colonies picked last night into 2x - W/0.1% glu

(300 ml 2x -W -U + 6ml ura + 1.5 ml 40% glucose) did not grow very well. Going ot use cultures from 13 July Monday.

1. pCTCON2:

   OD 660 = 1.370
   cells (107) = 3.64
   need cells (10^7) = 0.255 - 0.385
   dil = 11 x 
   get OD660 = 0.26

2. pCTCON2-GFP:

  OD660 = 1.357
  cells (107) = 2.58
  need cells (10^7) = 0.255 - 0.385
  dil = 11 x
  get OD 660 = 0.26

Actual OD 660 after dilution : pCT 0.168 pCT-GFP 0.171 11 a.m.

14:00 Very few cells in both but pCTCON2 does not glow and pCTCON2-GFP does, albeit not enough to photograph

15:30 More cells, but now neither glows.

End of the Day



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