IGEM:University of Chicago/2009/Notebook/Organophosphate Hydrolase/2009/07/02

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Entry title Insert your content here. July 2, 2009 Ago2 - presentation: constitutive or inducible promoter for opd Biosensor: GFP under paraoxon - sensitive promoter. p-nitrophenol biosensor: RFP under PNP-sensitive promoter Utilization?: Arthrobacter enzymes. Co-culture with Arthrobacter, expression of enzymes Biosensor - Paraoxon Continue + form assay - Tony Biosensor - PNP Continue + replace GFP with different color - Rob OPd display -Assay - how to determine surface localization? -cloning into yeast -longtine recombination -Promoters - contitutive, inducible, paraoxon PNP Degradation -Express each enzyme, be able to assay -2 people? Figure out expression, concerted activity End of Day