IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/10

From OpenWetWare

Jump to: navigation, search
University of Warsaw iGEM Team 2008 Main project page
Previous entry      Next entry

1. Plasmid isolation from previous day colonies.

2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI

3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and EcoRI

4. Control digest of pMPM-T5 + T7 RNA-polimerase with EcoRI

5. Control digest of pMPM-T5 + T7 RNA-polimerase with BamHI and XhoI

6. DNA electrophoresis of digestion products

7. Choose proper colonies of transformants



Personal tools