March 16th 2010
YFP (in vitro)
LovTAP
- We have a trouble with LovTAP system. Our system to repress and repressor is not 100% effective, because always is an expression independent of light reception.
General
- Efficiency of Quantum Yield could be possible in our system.
- There is an optional green light receptor and transcriptional response by same system.
We need to know
- Is there one way to work with LovTAP system?
- Best CDE genes of operon Lux.
- Luciferin-luciferase. How to be produced in E. Coli?
- How to anchor XFP on a Luciferase?
- What are the details of green light receptor?
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