IGEM:UNAM/2009/Notebook/IGEM project/2011/07/29

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Digestion

  • Today Vlad and me selected the best extraction of plasmid to do the digestion:
  • Selection of plasmid:

Gel 8. Plasmid(27/jul/11)

Line 1 -> J23110A
Line 2 -> J23110B
Line 3 -> J23110C
Line 4 -> J23110D
Line 6 -> J23111B
Line 7 -> J23111C
Line 10 -> J23112B
Line 11 -> J23112C
Line 13 -> J23113A
Line 14 -> J23113B

Gel 9. Plasmid (27/jul/11)

Line 4 -> J23114C
Line 9 -> J23115D
Line 11 -> J23116B
Line 12 -> J23116C
Line 13 -> J23116D

Gel 10. Plasmid (28/jul/11)

Line 1 -> J23117C
Line 3 -> J23118A
Line 4 -> J23118B
Line 5 -> J23118C
Line 6 -> J23118D
Line 7 -> J23119A
Line 11 -> J23100A
Line 12 -> J23100B
Line 13 -> J23100C
Line 14 -> J23100D
Line 15 -> J23101A

Gel 11. Plasmid (28/jul/11)

Line 3 -> J23101B
Line 4 -> J23101C
Line 5 -> J23101D
Line 6 -> J23102A
Line 7 -> J23102B
Line 9 -> J23102D
Line 10 -> J23103A
Line 11 -> J23103B
Line 12 -> J23103C
Line 13 -> J23103D
Line 15 -> J23104B

Gel 12. Plasmid (28/jul/11)

Line 1 -> J23104C
Line 2 -> J23104D
Line 3 -> J23105A
Line 4 -> J23105B
Line 5 -> J23105C
Line 6 -> J23105D
Line 7 -> J23106A
Line 8 -> J23106B
Line 9 -> J23106C
Line 10 -> J23106D
Line 11 -> J23107A
Line 12 -> J23107B
Line 13 -> J23107C
Line 14 -> J23107D
Line 15 -> J23108A

Reaction of the digestion:

  • Digestion
Reagent Quantity
H2O miliq 6 μl
Buffer 10X of EcoRI 1 μl
plasmid 2 μl
EcoRI enzyme 1 μl

Total 10 μL.




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