IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/17

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

Digestion test

I did the elctrophoresis gel to verify if the digestion was successeful:

  • Lane 1 Ladder 5kb
  • Lane 2 Digestion
  • Lane 3 pBBRMCS-5

With this gel I noticed that we did an incorrect digestion, because the lane 2 corresponds to the intact plasmid pS1T3 indicating that we tried to digest that palsmid, and we had to digest pBBRMCS-5. So is nedeed to repeat the digestion with the correct plasmid.



Personal tools