IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/06

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Plasmid isolation kit

  • I went with Dr. Miguel Ángel Ramírez to check the kit's reagents, he said that in general (smelling them) there was nothing bad, so perhaps the problem is more specific.

Transformation

I did the tranformation of:

  • The ligation of a double digestion: From it we expect to nothing grow, because if the double digestion was correct the ends generated wouldn't be adhesives
  • The ligation of nothing (control of the ligation).
  • Nothing (only competent cells).

The transformation was made according this protocol.


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