IGEM:UC/2006-6-7
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JL_06/07/06
Goal: We want to determine the antibiotics resistence of the F and R knockout plasmids (TlambdaOlambda)
Results:
We grew TlambdaOlambdaRlambda to early log density. We then streaked the cultures with pipette tips on LB agar plates containing the following anti-biotics:
- 15ug/mL gentamicin (G)
- 50ug/mL ampicillin (A)
- 25ug/mL chloramphenical (C) ---errr, axe that datapoint, the plate disapeared.--JCAnderson 13:45, 8 June 2006 (EDT)
- 50ug/mL spectinamycin (Sp)
- 25ug/mL tetracycline (Tet)
- 20ug/mL trimethylprim (Trim)
TlambdaOlambda grerw on kan only
Rlambda grew on kan and tet only--JCAnderson 19:07, 7 June 2006 (EDT)