Biofilm Track
Eric F. + Melody
Biofilm Growth Protocol Prep + Misc.
- Removed TSA plates from incubator, both showed plenty of individual colonies
- Made a large batch of 0.1% Crystal Violet (400mL sdH20 + 400microliters 100% CV)
Biofilm Growth Prtocol Day 4 - 100816E + 100816M
- Stained plates
- Melody stained with 0.01%, then removed and replaced with 0.1%
Biofilm Growth Protocol Day 5+ - 100811a-b(clean), 100811b-b(clean), 100814M(clean), 100814E(clean)
- Readings were better this time, see below
100811a-b(clean) - all wells
24 |
<> |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
25 |
A |
0.0839 |
0.0844 |
0.0843 |
0.0835 |
0.0839 |
0.0842 |
0.0841 |
0.0837 |
0.0838 |
0.0842 |
0.0842 |
0.0843 |
26 |
B |
0.0844 |
0.0846 |
0.0842 |
0.0845 |
0.0867 |
0.0843 |
0.0844 |
0.0847 |
0.0862 |
0.0846 |
0.0844 |
0.0849 |
27 |
C |
0.0846 |
0.085 |
0.0852 |
0.0868 |
0.0838 |
0.0831 |
0.0832 |
0.0839 |
0.0787 |
0.0846 |
0.0849 |
0.0847 |
28 |
D |
0.0849 |
0.085 |
0.0833 |
0.0794 |
0.0802 |
0.0845 |
0.0847 |
0.0753 |
0.0748 |
0.0763 |
0.0853 |
0.0852 |
29 |
E |
0.0855 |
0.0856 |
0.0843 |
0.085 |
0.0742 |
0.0893 |
0.0747 |
0.0826 |
0.0769 |
0.0853 |
0.0853 |
0.0858 |
30 |
F |
0.0853 |
0.0856 |
0.0888 |
0.0815 |
0.0842 |
0.0843 |
0.0777 |
0.0834 |
0.0873 |
0.0859 |
0.0857 |
0.0856 |
31 |
G |
0.0862 |
0.0857 |
0.0858 |
0.0856 |
0.0861 |
0.0859 |
0.0855 |
0.086 |
0.0863 |
0.0858 |
0.0861 |
0.0861 |
32 |
H |
0.0859 |
0.0871 |
0.0863 |
0.0867 |
0.0852 |
0.0853 |
0.085 |
0.0873 |
0.0855 |
0.0869 |
0.0859 |
0.0857 |
- Red = control
- Blue = nothing
100811b-b(clean) - all wells
24 |
<> |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
25 |
A |
0.084 |
0.0894 |
0.0797 |
0.0823 |
0.0798 |
0.0823 |
0.0791 |
0.0799 |
0.079 |
0.0804 |
0.0808 |
0.0879 |
26 |
B |
0.0843 |
0.0808 |
0.0813 |
0.0804 |
0.0806 |
0.0816 |
0.0808 |
0.0806 |
0.0807 |
0.082 |
0.0895 |
0.0808 |
27 |
C |
0.0881 |
0.0834 |
0.083 |
0.0812 |
0.0811 |
0.0804 |
0.0808 |
0.0811 |
0.0859 |
0.0821 |
0.0926 |
0.0831 |
28 |
D |
0.0877 |
0.0809 |
0.0848 |
0.0813 |
0.0838 |
0.0828 |
0.0805 |
0.0898 |
0.0844 |
0.0815 |
0.081 |
0.1112 |
29 |
E |
0.0876 |
0.0844 |
0.0822 |
0.081 |
0.0814 |
0.0827 |
0.0823 |
0.1048 |
0.0806 |
0.0849 |
0.0813 |
0.0876 |
30 |
F |
0.0867 |
0.0826 |
0.0822 |
0.0827 |
0.0821 |
0.0824 |
0.0812 |
0.0856 |
0.0828 |
0.0813 |
0.081 |
0.0818 |
31 |
G |
0.0826 |
0.0807 |
0.0816 |
0.0813 |
0.0822 |
0.0823 |
0.0854 |
0.1059 |
0.0839 |
0.0812 |
0.0813 |
0.0846 |
32 |
H |
0.0878 |
0.0845 |
0.0818 |
0.0821 |
0.0845 |
0.087 |
0.082 |
0.0858 |
0.0809 |
0.0829 |
0.0828 |
0.0923 |
100814M(clean) - all wells
25 |
<> |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
26 |
C |
0.0916 |
0.1647 |
0.2067 |
0.1649 |
0.1974 |
0.1989 |
0.25550 |
0.0992 |
27 |
D |
0.1602 |
0.1637 |
0.1574 |
0.2757 |
0.1035 |
0.2133 |
0.2133 |
0.2165 |
28 |
E |
0.1566 |
0.1763 |
0.1687 |
0.1928 |
0.1179 |
0.25710 |
0.2503 |
0.2387 |
29 |
F |
0.0986 |
0.2258 |
0.2215 |
0.2307 |
0.2611 |
0.249 |
0.2231 |
0.1006 |
100814E(clean) - all wells
25 |
<> |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
26 |
C |
0.0908 |
0.1277 |
0.1199 |
0.1093 |
0.1149 |
0.1089 |
0.1027 |
0.0901 |
27 |
D |
0.1168 |
0.1516 |
0.1478 |
0.0887 |
0.0887 |
0.1142 |
0.1094 |
0.107 |
28 |
E |
0.1165 |
0.1439 |
0.1367 |
0.0897 |
0.0899 |
0.1176 |
0.1731 |
0.1285 |
29 |
F |
0.1872 |
0.1806 |
0.1756 |
0.088 |
0.102 |
0.2068 |
0.1996 |
0.1745 |
|