IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/03

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Modified factors from protocol:

  • Bacterial:Broth = 1:50 dilution (500ul:
  • Aerated in using finlay Lab shaker (~90 rpm)
  • Aerated using 125ml flasks instead of 300ml flasks
Growth Curve Experiment
Time Sample 1 Sample 2 Sample 3 Sample 4
11:34-11:54am0.047-0.083-0.255 -0.345
11:34-11:54am0.059-0.070-0.259-0.343
12:00-12:30pm0.1780.0040.1910.107
12:00-12:30pm0.1900.0000.2000.000
12:30-1:00pm0.165-0.0750.2180.000
12:30-1:00pm0.170-0.0720.2250.000
1:10-1:40pm0.319-0.0830.462-0.004
1:10-1:40pm0.331-0.0920.467-0.002
1:50-2:20pm0.724-0.060.9330.006
1:50-2:20pm0.727-0.060.9510.002
Legend
SampleMixture
1staph+B2
2B2 Only
3LB +Staph
4LB only
5B2 Blank
6LB Blank


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