IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/24

iGEM Project name 1

Main project page Previous entry      Next entry

Tuesday 24th August: Transformations failed. Ordered competent DH5-alpha e.coli from Bioline. Set up an overnight restriction digest of pgaA promoter with EcoR1 and Pst1 restriction enzymes: Plasmid- 10μL Pst1 - 2μL EcoR1 - 2μL EcoR1 buffer - 2μL BSA (x20) - 1μL H2O - 3μL Ran an electrophoresis gel[[1]] of plasmids pACYC duet-1 and pCDF. Results shown bellow: The first three wells from the bottom were filled with the cut pACYC duet-1. The three wells between the two ladders contain the cut pCDF. pACYC duet-1 was extracted from the gel.[[2]]