IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/23

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Monday 23rd August: Transformation of pga promoter was successful. Transformations of GFPs and e. Chromi failed and repeated. Set up an overnight restriction digest of plasmids pACYC-duet, pCDF and pCOLA using Hind3 and Nco1 restiction enzymes: Plasmid- 10μL Hind3 - 2μL Nco1 - 2μL buffer - 2μL BSA (x20) - 1μL H2O - 3μL