IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/08/08

From OpenWetWare

Jump to: navigation, search
Bacterial eyespot Main project page
Previous entry      Next entry

Day 26

Today we did a miniprep with the IAIB precultures from yesterday We got poor results from 14ng/μL to 36,6ng/μL DNA on the tubes We runned simple digested samples on an agarose gel but we couldn't discriminate the difference between the vector only and the IAIB+vector samples due to a too small deferences We'd have to run a more concentrated gel

We then tried the assembly of IAIB ICIE IIAIIB IIC(1)5B We digested the bricks and tested them on agarose gel

As IB digestion didn't worked we did and tested it again

We then transformed the ligations into two new competent cells batches

One heat shock competent DH10B stock and one heat shock competent DH5α stock

We also tested the competence of the two new stocks with 1μg pBlueScript

Personal tools