IGEM:Stanford/2009/Notebook/Personal Notebook/2009/08/17

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Daily Work

Goals

Project / Accomplishments

  • Finished another revision for Construction Diagrams
  • Worked through Assay w/ PQ again....
    • 9am rough exp. w/ concentrated cells and PQ
    • 10am Y2E2 for diluted cells w/ varying PQ treatment, controls, and incubation
    • 1pm-3pm- plate reader


  • Designed New Primers and double-checked all before submittance


Problems

Alternative Solutions

Events

  • 1:15 meeting w/ Clinicial (w/ Ansuya and Jerome)
    • CANCELLED
  • Assay 1 for GFP w/ SoxR/S due to PQ
  • Wrong Gene Sequences Discovered!!

Comments

Weekly Goals

  • Figure out Assay- work more on proper controls
  • Keep revising diagrams and presentation w/ Anusuya


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