IGEM:Penn State University 2006/calendar/2006-7-5

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Contents

Wednesday

Tony

To do today

*e-mail Randy about R0051 and E. coli strain
*make sequencing primers (gene-walking and reverse primers)
*help Lane with mini-preps, restrictions, gel purification, etc.- assemble LaqIq + motB construct

Accomplished from yesterday

*Sequencing files obtained, examined


Jon

To do today

  • restrict (4h) IIR, E0422, E0432, E0434 and run gel
  • resubmit primers and IIR+motB and motB for sequencing
  • annoy Lane by not inquiring about 96 well plate

Accomplished from yesterday

  • miniprep P0451 (from 2005 plate), IIR (from cold room plate), and R0010.motB.B0015 (from successful ligation)

Chris

To do today

*

Accomplished from yesterday

*


Joel

To do today

*inoculate at 11 AM for plate reading at 7PM. Plate reading for 24 hour culture.

Accomplished from yesterday

*


Ralph

To do today

*

Accomplished from yesterday

*


Lane

To do today

*mini-prep LacIq constructs
*restrict, ligate, transform (LacIq, B0015+MotB)
*innoculate Mary's quorum constructs
*do sequencing stuff

Accomplished from yesterday

*Restricted, gel purified, ligated, transformed Mary's GFP+AAV tagged quorum constructs
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