IGEM:Penn State University 2006/calendar/2006-6-27

To Do: Tuesday

Tony

• restrict and run gel pSB4A3 (need more volume than yesterday)
• gel extract, ligate, and transform IIR+motB constructs into pSB4A3
• competency prep on RP437
• transform RP437 and RP3087 with GFP
• transform R0100+motB, C0051 (from registry plate) and pSB4A3 into DH5a

Jon

• use Pat's program to create a map of the IIR+motB part in pSB1A2 and pSB4A3
• restrict R0051 and motB, and run on gel
• make LB AND SOB

Joel

• Inoculate 6ml of inducible, 2ml constitutive, 2ml DH5-alpha at 11AM.
• Plate reading on 9hr cultures at 8PM and 24 hr cultures at 10PM.
• Read Drew Endy's plate reading assay protocals Endy lab page
  • inoculate 2ml of inducible (I14033 and I14032) and 2ml of constitutive.

Ralph

• Plate read (Pat's Lab) see above
• Read Drew Endy's plate reading assay protocals Endy lab page

Lane

• Innoculate colonies from p_LacIq+B0030/31/34+C0012 plates
• ?competency prep on RP437?
• Transform I14032+E0240 into RP437

Deepti

• stuff